【摘要】 采用绿色糖单孢菌为实验材料,在不同诱导产酶培养基上经过192h的振荡培养,探索其产酶时程规律.结果表明,不同的诱导底物诱导产生的木聚糖酶活性差异不显著,但诱导产纤维素酶活性差异显著.其中松木粉加棉纱培养基诱导产纤维素酶活性为0.08IU/ml,与空白对照(5.40IU/ml)相比显著下降(P≤0.05).为了适应纸浆漂白实际应用中纤维素酶越少越好的要求,选择该培养基为最佳诱导产酶培养基.绿色糖单孢菌在上述培养基中培养156h后达到木聚糖酶产酶高峰,粗酶液酶活可达到9.03IU/ml.通过对该酶进行高温及碱性处理,实验结果表明绿色糖单孢菌分泌的木聚糖酶在pH7.0下反应表现最高活性,同时在90℃下保温3h后酶活为原来的63.55%,具有较好的耐碱耐热性.更多还原

【Abstract】 Time course of xylanase’ s production was studied by culturin g Saccharomonospora viridis in different substrates for 192h.The results showed that xylanase demonstrated almo st the same activities in different substrates,while cellulase demonstrated different activities.Of them all the activity of cellulase inducted by pine wood powder +cotton yarn cultur e medium was 0.08IU /ml,and was drama tically different compared with the empty sample(5.40IU /ml )(P≤0.05).To meet the requirements for the actural environment of pulp bleaching,This culture medium was s elacted as the best substrate.After Saccharomonospora viridis having been cultured for 156h in the optimal enzy me-induction medium,the productio n of xylanase attained the high peak,with its maximal enzyme activity of 9.03IU /ml.The experiment results su ggested that the xylanase from Saccharomonospora viridis had the characteristics of both thermotolerance and alkali-tolerance,showing the remaining 63.55%of its maximal activity under treatment for 3h at 90℃,an d the highest activity in function in pH 7.0,respectively.更多还原