【摘要】 ADAMs家族是含多结构域的跨膜蛋白。睾丸特异的ADAMs,在精子发生与附睾精子转运过程中,经过蛋白水解成为成熟精子的分子形式,与精-卵质膜结合和融合有关。对于精-卵质膜相互作用,ADAMs去整合素域具有关键氨基酸残基和特殊模体。模拟ADAM2和ADAM3去整合素域的短肽能用于鉴别特异性卵子识别蛋白。精子ADAMs去整合素域与卵子膜蛋白整合素β1、α4/α9、α6和CD9相互作用,介导了精卵质膜的结合与融合。更多还原

【Abstract】 The ADAMs (A disintegrin and A metalloprotease)family are multidomain proteins.Some ADAMs appear to be testis-specific and have been shown to be proteolytically to form functional moleculars on mature sperm during spermatogenesis and epididymal transport. The testis-specific ADAMs may play a role in sperm- egg plasma membrane adhesion and fusion. It is necessary that the disintegrin domain of ADAMs contain some key amino acids and specific motif for sperm-egg interaction. The short synthetic peptides, based on the disintegrin-like domains of ADAM2 and ADAM3 can be used in identifying specific oocyte recognition proteins. It has been suggested that the disintegrin domain of sperm ADAMs can interact with integrinsβ1, α4/α9, α6, and β1 integrin-associated protein CD9 on the egg.更多还原