【摘要】 为了观察结状神经节与心肌细胞联合培养中神经元的迁移和肽类神经递质的合成,我们建立了Wistar大鼠迷走神经结状神经节与心肌细胞联合培养的模型。用倒置相差显微镜观察了结状神经节与心肌细胞联合培养不同时间的活细胞生长情况。用Holmes还原银染色法观察了联合培养72 h和96 h的标本中神经元和心肌细胞的生长以及神经元的迁移。用免疫组织化学法观察了联合培养72 h和96 h的标本中肽类神经递质—P物质(SP)和降钙素基因相关肽(CGRP)的合成。结果表明,结状神经节与心肌细胞联合培养中,神经元在形态学上的发育成熟可能需要72 h就可以完成,而神经递质合成的发育成熟则需要96 h。神经元在形态学上的发育成熟并不能代表神经递质合成的发育已经完成。更多还原

【Abstract】 To investigate the neuronal migration and synthesis of peptidic neurotransmitters in vitro, neuromuscular co-cultures of nodose ganglia and cardiac muscle cells of Wistar rats were established. The living cells of co-cultures were observed with an inverted contrast microscope at different culture age. At 72 h and 96 h of culture age, the neuromuscular co-cultures were examined by Holmes’ reduced silver staining technique for observing the neuronal migration. Immunohistochemical staining method was used for investigating the synthesis of peptidic neurotransmitters-substance P (SP) and calcitonin gene-related peptide (CGRP). The neuronal morphorlogical maturation in neuromuscular co-cultures seems at 72 h of culture age in terms of the observation of living cells and Holmes’ reduced silver staining technique. SP- and CGRP-immunoreactive neurons were observed in neuromuscular co-cultures at 96 h of culture age but not 72 h. The results indicate that the neuronal morphological maturation can not represent the maturation of neurotransmitter synthesis. The synthesis of neurotransmitters is not maturated in the neuromuscular co-cultures until 96 h of the culture age.更多还原