【摘要】 目的优化色谱条件,在最佳条件下对蒙山九州虫草和冬虫夏草子座中核苷类化合物进行定性和定量研究,并比较两种虫草的不同。方法用高效液相色谱(HPLC)法对供试品中核苷类成分进行初步定性和定量的研究,然后用质谱(MS)法进行成分验证。结果HPLC与MS法联用能准确地对各组分进行定性和定量分析;洗脱液的最佳配比为:先以99%水+1%乙腈梯度洗脱5 min后过渡为96%水+4%乙腈至洗脱完全。结论首次将此方法用于虫草中核苷类成分的研究,发现蒙山九州虫草含有比冬虫夏草种类更多的核苷类成分,有望替代价格昂贵的冬虫夏草应用于临床。更多还原

【Abstract】 PurposeTo optimize the chromatogram condition,to study nucleotides in stroma of Cordyceps sinensis and Cordyceps kyushuensis,and to compare the nucleotide contents of the two Cordyceps sp.MethodsThe qualitative and quantitative nucleotides in the stroma of Cordyceps sp.were analysed by HPLC-MS.ResultsThe contents of the nucleotides in the samples were exactly identified and quantitated.The samples were ananlysed according to the following gradient program:0-5 min: from 99%H₂O+1%ACE to 96%H₂O+4%ACE,5-30 min:96%H₂O+4%ACE.ConclustionHPLC-MS is used for the first time to study nucleotide in stroma of cordyceps sp.Cordyceps kyushuensis has good prospect,and can also be expected to replace Cordyceps sinensis clinically.更多还原