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胰胆合流异常胆汁和血清淀粉酶的二维电泳

Amylase Isoforms in the Bile of Patients with Pancreaticobiliary Malunion Analysed by Two-Dimensional Electrophoresis

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【作者】 龚振华肖现民徐绍英张祥民

【Author】 GONG Zhen-hua,XIAO Xian-min,XU Shao-ying,ZHANG Xiang-min Department of Surgery, Children ’ s Hospital, Fudan University, Shanghai 200032;Department of Surgery, Shanghai Children’ s Hospital, Shanghai Jiaotong University, Shanghai 200040; Department of Chemistry, Fudan University, Shanghai 200433, China

【机构】 上海交通大学附属上海市儿童医院外科复旦大学附属儿科医院外科复旦大学化学系复旦大学化学系 200040上海 200032上海 200433上海 200433

【摘要】 目的 通过二维电泳分析胰胆合流异常(PBM)患者的胆汁和血清淀粉酶的等电点和相对分子质量 (Mr)。方法 通过醋酸纤维素薄膜电泳,将正常人血清和PBM患者胆汁和血清中淀粉酶初步分离提纯,继经 等电聚焦和SDS-PAGE二维电泳,测定淀粉酶同工酶的Mr和等电点。结果 在醋酸纤维素薄膜电泳中,淀粉 酶泳动在γ球蛋白负极侧,显示出2条谱带。在二维电泳中,正常人血清淀粉酶的2个同工酶分别是等电点 p16.4(Mr≈54×103)和等电点p16.7(Mr≈55×103)的两个点:PBM患者胆汁和血清中,存在6个淀粉酶同工酶 点,除了上述正常人血清淀粉酶的两个主要点之外,在p16.8和p17.2分别出现Mr≈55×103两个新点,在p16. 0和p16.2分别出现Mr≈53×103-54×103的两个新点。结论 在PBM患者胆汁和血清中,淀粉酶存在6种 同工酶,这一系列同工酶的Mr都接近正常的55×103,而等电点分布在p16.0到p17.2之间的6个点上,而正常 人血清淀粉酶只存在两种同工酶。

【Abstract】 Purpose To determine the isoelectric points (pI) and molecular masses (MS) of amylase isoforms in the bile and serum of patients with pancreaticobiliary malunion (PBM) . Methods We isolated amylases in serum of normal and serum and bile juice of PBM patients using cellulose acetate membrane electrophoresis, then analysed the pI and MS of amylase isoforms by two-dimensional electrophoresis. Results The amylase ran after γ-globin on cellulose acetate membrane electrophoresis and presented in two bands. There were about six plots of amylase isoforms in the bile juice and serum of patients with PBM, which had almost the same MS of 55 000,but with different pI ranging from 6.0 to 7. 2 in two-dimensional electrophoresis. There we’re two plots of isoforms in normal serum. Conclusions There were more amylase isoforms in the bile juice and serum of patients with PBM, which had almost the same MS of 55 000, but with different pI ranging form 6.0 to 7.2. There were two amylase isoforms in normal serum.

  • 【文献出处】 复旦学报(医学版) ,Journal of Shanghai Medica(University) , 编辑部邮箱 ,2005年01期
  • 【分类号】R446.11
  • 【被引频次】2
  • 【下载频次】102
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