【摘要】 将人白细胞介素24(hIL24)的cDNA序列克隆至原核高效表达载体pET21a(+)上,经IPTG诱导后,在大肠杆菌中获高效表达,经纯化、复性后的rhIL24蛋白具有抑制HeLa细胞生长,诱导HeLa细胞凋亡,刺激外周血单核细胞分泌IL6、TNFα、IFNr,抑制血管形成的功能。初探了rhIL24蛋白能通过下调抗凋亡因子bcl2表达,激活线粒体凋亡途径引起肿瘤细胞凋亡的机理。更多还原

【Abstract】 The hIL24 cDNA sequence was cloned into prokaryotic high expressive vector pET-21a(+) and recombinant hIL24 was expressed in E. coli with IPTG induction. The purified recombinant hIL24 exhibits following functions in HeLa cell: inhibiting cell growth, inducing apoptosis, inducing PMBC to secrete IL-6、TNF-α、IFN-r and inhibiting blood vessel formation. Our preliminary results suggest that the apoptosis induced by rhIL24 is through down-regulating expression of anti-apoptosis factor Bcl-2 and activation of mitochondria apoptosis pathway.更多还原