Function of c-Fos-like and c-Jun-like Proteins on Trichostatin A-induced G₂/M Arrest in Physarum polycephalum

【摘要】 <正> The homologs of transcription factors c-Fos and c-Jun have been detected in slime moldPhysarum polycephalum during progression of the synchronous cell cycle.Here we demonstrated that c-Fos-like and c-Jun-like proteins participated in G₂/M transition by the regulation of the level of Cyclin B1protein in P.polycephalum.The study of antibody neutralization revealed that interruption of the functions ofc-Fos-like and c-Jun-like proteins resulted in G₂/M transition arrest,implicating their functional roles in cellcycle control.When G₂/M transition was blocked by histone deacetylase inhibitor trichostatin A,changes inc-Fos- and c-Jun-like protein levels,and hyperacetylation of c-Jun-like protein,were observed.The datasuggest that in P.polycephalum,c-Fos- and c-Jun-like proteins may be the key factors in the regulation ofhistone acetylation-related G₂/M transition,involving the coordinated expression and hyperacetylation ofthese proteins.更多还原

【Abstract】 The homologs of transcription factors c-Fos and c-Jun have been detected in slime mold Physarum polycephalum during progression of the synchronous cell cycle.Here we demonstrated that c- Fos-like and c-Jun-like proteins participated in G₂/M transition by the regulation of the level of Cyclin B1 protein in P.polycephalum.The study of antibody neutralization revealed that interruption of the functions of c-Fos-like and c-Jun-like proteins resulted in G₂/M transition arrest,implicating their functional roles in cell cycle control.When G₂/M transition was blocked by histone deacetylase inhibitor trichostatin A,changes in c-Fos- and c-Jun-like protein levels,and hyperacetylation of c-Jun-like protein,were observed.The data suggest that in P.polycephalum,c-Fos- and c-Jun-like proteins may be the key factors in the regulation of histone acetylation-related G₂/M transition,involving the coordinated expression and hyperacetylation of these proteins.更多还原