【摘要】 光动力疗法(PDT)已发展成为一种较成熟的肿瘤治疗方法,PDT诱导的血管损伤是杀死肿瘤的重要机制之一.为了在活体肿瘤模型上实时监测PDT导致的血管损伤效应,使用稳定高表达绿色荧光蛋白(GFP)的人涎腺腺样囊性癌细胞株(ACC-M-GFP),建立了基于鸡胚尿囊膜(CAM)的肿瘤模型.应用荧光成像技术对肿瘤的生长位置、大小,以及治疗区域进行方便精确的定位;利用激光散斑成像技术,实时监测CAM上肿瘤周围血管的血液动力学参数.发现不同光动力剂量所导致的血管损伤有显著不同.结果表明,荧光标记的鸡胚尿囊膜肿瘤模型为研究PDT导致的血管损伤效应提供了良好的实验模型,激光散斑成像技术适用于实时监测PDT过程中血管结构、血流速度的变化,由此得出血液灌注率可用以评估PDT对肿瘤周围血管的损伤效应.更多还原

【Abstract】 Photodynamic therapy (PDT) has been widely used as an anticancer treatment in the clinic. However, the damage to the vessel induced by PDT, one of the most important aspects to tumour therapy, is not clearly understood. In order to study the effect of vascular damage in vivo during PDT, the chicken chorioallantoic membrane (CAM) tumor model by using stable high GFP-expressing human adenoid cystic carcinoma cell strain (ACC-M-GFP) is built for the experiment. The fluorescence imaging was used to show the location and the size of the tumor and treatment region. The dynamic parameter change of blood with different photodynamic doses in PDT was monitored in real time by laser speckle contrast imaging (LSCI) technique. The experimental results show that: the photodynamic effects are observably different at different photodynamic doses. It proved that: firstly the GFP-expressing tumor CAM model is extremely suitable for studying the photodynamic effect on the vessels, because of its convenience to locate the tumor and treatment region on the CAM. Secondly, with the aid of the LSCI technique, it is able to perform real-time monitoring of vessel structure and flow velocity change to identify the perfusion variation in PDT and to additionally investigate the damage to the vessels around the tumor induced by PDT.更多还原