【摘要】 P15RS是在p15INK4b高表达的人黑色素瘤细胞MLIK6G1期中发现和克隆的新基因.研究表明,该基因可能在G1期作为增殖负调因子起作用.为了观察P15RS基因产物在细胞中的定位,构建了表达P15RS-EGFP融合蛋白的真核表达质粒pEGFP-P15RS.用绿色荧光蛋白GFP作为报告基因,以人胃癌细胞BGC-823为实验模型,将pEGFP-P15RS转染BGC-823细胞.实验表明,P15RS基因表达产物在G1期、S期和G2期均定位于细胞核内,未见在核仁中分布,M期凝缩的染色体上未见.因此,P15RS可能定位在核质中.更多还原

【Abstract】 P15RS was identified as a novel gene cloned from the human melanoma cell model MLIK6 overexpressing p15INK4b. P15RS has been reported as a negative regulator of cell proliferation in G1 phase. To investigate the localization of P15RS, EGFP-P15RS, a fusion protein with EGFP reporter was constructed and overexpressed in BGC-823 cells. The results showed that EGFP-P15RS protein was distributed in nuclear in interphase (G1, S, G2 phase) of BGC-823 cells. The EGFP-P15RS protein was observed not to localize in chromosome in M phase, suggesting that P15RS might be located in karyoplasm.更多还原