【摘要】 制备了鳗弧菌E3-11菌株的兔抗血清,并应用间接酶联免疫吸附法,建立了一种新的快速诊断鳗弧菌的方法。该方法能检出⒈5×105个·ml-1浓度的E3-11菌株,对患病鱼体的组织培养物鳗弧菌进行检测,呈现明显的阳性反应。对嗜水气单胞菌(A.hydrophila),柱状纤维粘细菌(C.columnaris),非01霍乱弧菌(Non-01)等几种在养殖中主要致病菌不发生交叉反应,同时该反应过程可在较短时间内完成。该方法具有敏感性高、特异性强、速度快、实用方便等特点,可应用于鳗弧菌的快速检测和诊断。更多还原

【Abstract】 A new method for the rapid diagnosis of vibiosis was established by preparing rabbit antiserum for E3-11 strain of V. anguillarum, and indirect ELISA . By this method E3-11 strain at the concentration of 1.5×105 ind.·ml-1. was able to be detected and there were obvious positive reaction when V. anguillarum in the tissue culture of affected fishes was checked. The method did not have cross reaction to several pathogenic bacteria during the culture, e.g., Aeromonas hydrophila , Cytophaga columnaris ,Non-01 comma bacillus, and the reaction would be completed in a short time. Conclusion this method has high sensibility, high singularity and high speed as well as easy usage and can be utilized in rapid detection diagnosis of vibiosis.更多还原