外周血单个核细胞FcγRⅡb和血清C₁q抗体变化在SLE发病机制中的作用

【摘要】 目的探讨外周血单个核细胞FcγRⅡb及血清C1q抗体的表达在系统性红斑狼疮(SLE)发病机制中的作用。方法检测了41例SLE患者和30例正常人外周血粒细胞、淋巴细胞和单核细胞FcγRⅡb的表达及血清C1q抗体水平,将上述指标分别与抗核抗体(ANA)、dsDNA抗体及SLE活动性指数评分(SLEDAI)作相关性分析。结果SLE患者外周血粒细胞、淋巴细胞和单核细胞FcγRⅡb的表达均减少(以粒细胞和单核细胞为主),血清C1q抗体水平明显增高,FcγRⅡb和C1q抗体与ANA、抗dsDNA及SLEDAI评分分别呈负相关和正相关;FcγRⅡb与C1q抗体呈低-中度负相关。结论外周血单个核细胞FcγRⅡb表达缺陷和血清C1q抗体水平升高在SLE的免疫发病机制中起重要作用;FcγRⅡb和C1q抗体是判断SLE病情活动性的重要指标。更多还原

【Abstract】 Objective: To investigate the effect of FcγRⅡb and C₁q in PBMC on the pathogenesis of SLE.Methods: FcγRⅡb of granulocyte、lymphocyte、monocytes taken from peripheral blood in 41 SLE patients and 30 health control were detected by flow cytometry,C₁q in serum were measured by ELISA.FcγRⅡb and C₁q Antibody were compared with ANA、dsDNA and SLEDAI score,a dependability between them was analyzed.Results: Expression of FcγRⅡb in granulocyte、lymphocyte、monocytes of SLE PBMC decreased,especially in granulocyte and monocytes.C₁q antibody in serum increased obviously.FcγRⅡb had a negative correlation with ANA、dsDNA and SLEDAI,but C₁q had a positive correlation with them.FcγRⅡb got a low-midrange negative correlation with C₁q antibody.Conclusion: The defection of FcγRⅡb expression in SLE patients and the high level of serum C₁q antibody decreasing the function of cleaning the immune comples.FcγRⅡb and C₁q play an important role in SLE pathogenesis and judging condition and reactiveness of SLE.更多还原