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HPLC法对不同产地北豆根中蝙蝠葛碱的测定

Determination of Dauricine in Rhizoma Menispermi from different dist ricts by HPLC

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【作者】 于维萍傅春升陶琳璐

【Author】 Yu Wei-ping,Fu Chun-sheng,Tao Lin-lu(Teaching Hospital of Shandong University of Traditional Ch inese Medicine,Ji’nan,250011)

【机构】 山东中医药大学附属医院山东中医药大学附属医院 济南250011济南250011济南250011

【摘要】 目的 研究不同产地北豆根中蝙蝠葛碱含量的差异。方法 以北豆根饮片为分析对象,用phenomenexC18(4 . 6mm×15 0mm,5 μm)色谱柱,采用乙腈和0 . 2 %磷酸洗脱,流速1. 0ml·min-1,检测波长为2 84nm,对北豆根中的蝙蝠葛碱进行定性定量测定。结果 东北北豆根药材中生物碱含量最高,其次是山东产北豆根,河北产北豆根药材中蝙蝠葛碱含量较低。结论不同产地北豆根药材所含生物碱在质和量两方面均有较大差异。

【Abstract】 Objective To study the difference of Daurici ne i n Rhizoma Menispermi from different districts.Methods HPLC an alysis method of Rhizoma Menispermi was studied and the chromatographic conditi on was as the following:phenom enex C 18(4.6mm×150mm,5μm)column;flow rate:1.0ml·min -1;mobi le phase:0.2%H 3PO 4 solution and CH 3CN;wavelength:284nm.Results The content of Dauricine in Rhi zo ma Menispermi from the North of China was highest,and the content of Dauricine in Rhizoma Menispermi from Hebei is low.Conclusion Considerable difference was detected among the several crude drugs from different districts.

【关键词】 北豆根HPLC蝙蝠葛碱
【Key words】 Rhizoma MenispermiHPLCDauricine
  • 【文献出处】 齐鲁药事 ,Shangdong Pharmaceutical Industry , 编辑部邮箱 ,2005年03期
  • 【分类号】R284.1
  • 【被引频次】10
  • 【下载频次】254
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