【摘要】 目的:探讨中药颤复宁血清对6羟基多巴(6OHDA)诱导的大鼠嗜铬细胞瘤细胞(PC12)凋亡的抑制作用。方法:用血清药理学方法制备中药血清,加到经6OHDA处理的PC12细胞培养液中,用四甲基偶氮唑蓝(MTT)法检测细胞存活率,并用免疫组织化学染色方法和图像分析检测酪氨酸羟化酶(TH)含量及半胱氨酸蛋白酶3(Caspase3)阳性凋亡细胞数。结果:颤复宁中药血清能有效地提高PC12细胞的存活率,对6OHDA诱导的凋亡有明显抑制作用。与6OHDA损伤组比较,颤复宁血清组TH阳性细胞平均吸光度增高(P<0.01),Caspase3阳性细胞数明显降低(P<0.01)。结论:中药颤复宁血清对PC12细胞具有直接的神经营养作用,能提高细胞存活率,且对6OHDA诱导的细胞凋亡具有抑制作用。更多还原

【Abstract】 Objective: To study the inhibitory effect of chanfuning serum on the apoptosis of PC12 cells induced by 6-hydroxydopamine (6-OHDA). Methods: Serum pharmacological method was used to make chanfuning serum. 6-OHDA and chanfuning serum were added into the medium of PC12 cells separately. MTT method was used to assay the cell’s viability, and tyrosine hydroxylase (TH) and caspase-3 immunocytochemistry staining were used to evaluate the content of TH and count the number of apoptosis cells. Results: PC12 cell’s viability and calibrated optic density of TH positive cells enhanced (P<0.01), and the number of caspase-3 positive cells decreased(P<0.01) significantly in chanfuning serum treated group compared with 6-OHDA treated group. Conclusion: Chanfuning serum may have direct nutritious effect, protect PC12 cells from injury induced by 6-OHDA, and produce anti-apoptosis effect to some extent.更多还原