【摘要】 以虹鳟二倍体的伪雄鱼为父本(遗传型为xx),探讨了采用热休克方法阻止第二极体排放诱导全雌虹鳟三倍体的适宜条件。结果表明:虹鳟三倍体诱导率明显受处理温度、起始时间以及持续时间等因子的影响。在孵化水温为6.5℃,卵子受精后20min经26℃热处理20min,孵化率为64.62%,三倍体诱导率为86.67%;卵子受精后10min经26℃热处理20min,三倍体诱导率为100%,但孵化率仅为3.87%。受精卵经26℃处理的诱导效果好于24℃和28℃的(P<0.01)。更多还原

【Abstract】 Sex reversed diploid rainbow trout whose genotype was XX was as father,heat shock was used to restrain the second meiosis of the fertilized eggs of rainbow trout to induced all female rainbow trout triploid.It trued out that the all female rainbow trout triploid induction rate was obviously affected by the treatment temperature,the beginning and continuing treatment time and so on.The triploid induction rate could reach 86.67% and the hatching rate could reach 64.42% at 20min after fertilization with 26℃ heat shock as the continuing treatment time was 20min in 6.5℃ water.and at 10min after fertilization with 26℃ heat shock as the continuing treatment time was 20min,the triploid induction rate could reach 100%,but the hatchability was 3.87%.The induction rate of 26℃ was better than 24℃ and 28℃.更多还原