【摘要】 采用聚丙烯酰胺凝胶电泳法对成年白香猪的肝、胰、小肠和大肠等脏器组织的乳酸脱氢酶(LDH)进行分离,结果显示:它们均含有5条LDH同功酶谱带。经生物图像处理系统扫描定量,得出LDH1、LDH2、LDH3、LDH4和LDH5各组分在肝脏中的百分含量顺序为:LDH5>LDH2>LDH1>LDH3>LDH4。M亚基∶H亚基为49.52%∶50.48%;在胰脏中的百分含量顺序是:LDH1>LDH2>LDH5>LDH3>LDH4,M亚基∶H亚基为32.86%∶67.14%;小肠中的百分含量排列依次是:LDH5>LDH4>LDH1>LDH2>LDH3,M亚基∶H亚基为63.18%∶36.82%;大肠中的百分含量次序是:LDH1>LDH5>LDH2>LDH4>LDH3,M亚基∶H亚基为42.00%∶58.00%。以上LDH同功酶在不同消化器官组织中各谱带及亚基的组成与含量的不同,反映了相应组织中的乳酸和丙酮酸间转化代谢过程的差异,由此保证了机体糖代谢中LDH所催化的反应的正常协调进行。更多还原

【Abstract】 The lactic dehydrogenase LDH isoenzyme of liver pancreas small intestine and large intestine were determined by polyacrylamide gel electrophoresis in adult(7-months) white bands mini-pig. The results showed that there were five LDH isoenzyme in these tissues. The order of LDH composed in the liver was LDH5>LDH2>LDH1>LDH3>LDH4, ratio of subunit M and subunit H was 49.52%∶50.48%. The order of LDH composed in pancreas was LDH1>LDH2>LDH5>LDH3>LDH4, ratio of subunit M and subunit H was 32.86%∶67.14%. The order of LDH composed in small intestine was LDH5>LDH4>LDH1>LDH2>LDH3, ratio of subunit M and subunit H was 63.18%∶36.82%.The order of LDH composed in large intestine was LDH1>LDH5>LDH2>LDH4>LDH3, ratio of subunit M and subunit H was 42.00%∶58.00%. All the results indicated that the LDH isoenzyme in different digestive organ tissues, such as liver,pancreas,small and large intestine reflected the diversity of metabolic characteristic in different muscle tissues so that ensured carbohydrate metabolism in order.更多还原