【摘要】 对双相氯胺-T碘标法实验装置作了改进并标记长效促红细胞生成素(LL-EPO),采用离心超滤技术代替传统的柱层析脱盐纯化标记蛋白质。结果显示125I-LL-EPO具有高比活性、高生物活性、高回收率。说明双相氯胺-T碘标法简单方便,是蛋白质同位素碘标记的理想方法;离心超滤技术适合放射性碘标记蛋白质的纯化。更多还原

【Abstract】 This research presented a modified two-phase chloramine-T method for radioiodine labeling of protein and Long-lasting Erythropoietin(LL-EPO) was labeled.And traditional gel filtration was substituted by ultrafiltration to purify the labeled protein.The results show that labeled protein possesses high specific radioactivity,biological activity and recovery.It suggests that two-phase chloramine-T method is simple and convenient and an ideal method to iodinate protein.Ultrafiltration is suitable for purification of the radioiodine labeled protein.更多还原