【摘要】 在实验条件下,将僧帽牡蛎分别置于含有0.5、2、5 mg·L-10号柴油水溶性成分的海水中,在污染后1、4、7、11、15 d采样,15 d后转入清洁海水中进行6 d的恢复实验,采样,观察消化腺和鳃脂质过氧化的程度。结果表明:(1)正常生理条件下消化腺和鳃也有一定程度的脂质过氧化,消化腺显著高于鳃;(2)脂质过氧化的程度随污染时间的延长而增加,并存在一定的剂量-效应关系,可作为监测海洋石油污染的生物标志物;(3)解除污染后,脂质过氧化恢复到对照水平。更多还原

【Abstract】 Lipid peroxidation is a process in which the polyunsaturated fatty acid components of lipids are oxidized to form free radicals and lipid peroxide metabolites, therefore capable of causing extensive tissue damage. Lipid peroxidation maybe has more important meaning to aquatic organism than to other organism, because of the abundant polyunsaturated fatty acid in aquatic organism. This paper is the first report about the effect of oil pollution on lipid peroxidation in marine bivalve from China. In laboratory condition, oyster, Ostrea cucullata was exposed to different concentrations of water-soluble fraction (WSF) of No.0 diesel oil (0.5,2,5 mg· L-1) for 15 d, and then transferred into clean sea water for 6 d restoring experiment. The lipid peroxidation (determined as malondialdehyde levels, MDA) in digestive gland and gill of 1,4,7,11,15,21 d samples were measured, the results showed: (1) Lipid peroxidation also occurred in digestive gland and gill of control group, and the former was higher than the latter; (2) In digestive gland sample of 0.5,2,5 mg·L-1 group, the significant enhancement of b’pid peroxidation in comparison with control group appeared in 15,11,4 d respectively, and the increases of lipid peroxidation were observed with the prolonged exposure time, and a dose-effect relationship was occurred in 15 d sample; there was a similar trend of change in gill lipid peroxidation, but less obvious than that of digestive gland; (3) Lipid peroxidation of all concentration groups returned to control level after recovery experiment. According to our data, the lipid peroxidation of marine bivalve can be a biomarker to marine oil pollution, and that needs further research.更多还原