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T95对灰葡萄孢的拮抗作用及田间防病研究

THE STUDY ON ANTAGONISM OF TRICHODERMA SP·AGAINST BOTRYTIS CINEREA AND BIOLOGICAL CONTROL

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【作者】 孙芙蓉吴悦明徐玉芳张修国张福进

【Author】 SUN Fu-rong1,WU Yue-ming 2* ,XU Yu-fang3,ZHANG Xiu-guo2,ZHANG Fu-jin4 (1.Entry-Exit Inspection-quarantine Bureau of Zibo,Zibo 255000,China; 2.Departnent of Plant Pathology Shandong AgriculturalUniversity,Taian 271018,China; 3.Vocational-technical College of Taian,Taian 271000,China; 4.Plant Protection Station of Huancui District of Weihai city,Weihai 264200,China)

【机构】 淄博出入境检验检疫局山东农业大学植保学院山东泰安职业技术学院威海市环翠区植保站 淄博张店255000山东泰安271018山东泰安271000山东威海264200

【摘要】 用对峙培养法和抑制孢子萌发法,筛选出1株对灰葡萄孢BotrytiscinereaPers.具有较强拮抗作用的木霉菌株T95,该菌株可产生大量较细的螺旋状分枝菌丝缠绕病菌菌丝,使其断裂并可迅速消解病菌的菌丝和孢子。孢子浓度为6×106/ml时,可有效抑制病菌孢子萌发。田间防病试验显示,T95孢子量为5×106/ml,喷雾2次,对冬季大棚番茄灰霉病的防效可达80.9%。

【Abstract】 Isolate T95 of Trichoderma sp. Isolated from soil has shown the antagonistic action against the pathogen of Botrytis cinerea Pers. In solid culture medium. Antagonistic action showed parasitism and competition between isolate T95 and the above pathogen. Effective were 6×106/ml conidia ; The control experiment in the fields showed when effective inhibitory concentrations of isolate T95 were 5×106/ml conidia,it was the most effective in greenhouse experiments to against Botrytis cinerea Pers. and it′s control efficiency was more than 80.9%.

【关键词】 木霉菌灰葡萄孢拮抗机制生物防治
【Key words】 trichodermabotrytis cinereaantagonishbiocontrol
  • 【文献出处】 山东农业大学学报(自然科学版) ,Journal of Shandong Agricultural University , 编辑部邮箱 ,2005年02期
  • 【分类号】S436.412;S476.1
  • 【被引频次】12
  • 【下载频次】149
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