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甘蓝型油菜(Brassica napus)中一个未知功能蛋白基因的cDNA克隆

The cDNA Cloning of a Gene Encoding an Unknown Protein from Brassica napus

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【作者】 邱峰王茂林刘阳周云涛赵云张帆

【Author】 QIU Feng,WANG Mao-lin,LIU Yang,ZHOU Yun-tao,ZHAO Yun,ZHANG Fan(1.College of Life science,Sichuan University,Chengdu 610064,China;2.Sichuan Provincial Seed Station,Chengdu 610041,China)

【机构】 四川大学生命科学学院四川省种子管理站 成都610064成都610064成都610041

【Abstract】 According to the sequence of a 352bp fragment-M17 from the suppression subtractive hybridization library of the mutant line Cr3529 of Brassica napus,primers were designed and a 1000bp upstream fragment was cloned by 5’-RACE,which was joined with the original piece and composed a 1343bp sequence.According to the 3’ and 5’ ends sequences,a pair of primers were designed and the encoding region sequence of this unknown protein was amplified and cloned.Sequence analyzing showed that it had 80.1% identity with an unknown-protein-encoding gene from Arabidopsis thaliana.Swiss-model was used to predict the structure of the induced protein,and a potential transmembrane helix from site 46 Leu to 66 Phe was identified.GLOBE prediction of globularity showed that this protein possessed a compact globular domain.

【关键词】 Brassica napusRACEunknown proteinprotein predict
【Key words】 Brassica napusRACEunknown proteinprotein predict
【基金】 国家自然科学基金资助项目(30170500)
  • 【文献出处】 四川大学学报(自然科学版) ,Journal of Sichuan University (Natural Science Edition) , 编辑部邮箱 ,2005年05期
  • 【分类号】S565.4;
  • 【下载频次】279
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