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Tsα1诱导人肝癌SMMC-7721细胞凋亡和抑制bcl-2基因的表达
Expression of apoptosis and inhibitory bcl-2 in human hepatocelluar cancer SMMC-7721 cells induced by thymope α1
【摘要】 目的:研究胸腺肽α1(Tsα1)在诱导肿瘤细胞凋亡中的作用。方法:应用MTT比色分析法研究Tsα1对人肝癌SMMC7721细胞株的细胞毒作用。流式细胞仪检测法分析Tsα1作用后处于G1期SMMC7721细胞的比率和对凋亡抑制基因bcl2蛋白的抑制率。结果:Tsα1(4000μg/mL)体外培养24、48和72h,对SMMC7721肿瘤细胞抑制率分别是(1.92±0.02)%、(3.27±0.03)%和(7.69±0.03)%,P<0.005;凋亡率分别是(2.5±0.1)%、(18.3±0.2)%和(11.6±0.1)%,P<0.001;bcl2蛋白的抑制率为(1.35±0.1)%、(2.2±0.01)%和(5.53±0.01)%,P<0.005,并呈现细胞凋亡的形态学改变,具有剂量和时间依赖性。结论:Tsα1可直接抑制人肝癌SMMC7721细胞株增殖,抑制bcl2基因表达和诱导肿瘤细胞凋亡。
【Abstract】 OBJECTIVE: To stude the apoptosis induced by thymope α1(Tsα1) in human hepatocelluar cancer SMMC-7721 cells. METHODS: Tsα1 inhibitatory SMMC-7721 cell’s growth by was studied MTT assay; morphological characteristics of apoptosis was observed by microscopy; cell apoptosis rate was measured by flow cytometry and agarrose gel electronphoresis; expression of bcl-2 was detected by flow cytometry. RESULTS: When the concentration of Tsα1 was 4 000 μg/mL, the inhibitatory rates of SMMC-7721 cells were (1.92±0.02)%, (3.27±0.03)% and (7.69±0.03)% at 24 h, 48 h and 72 h, respectively,P<0.005. The apoptosis rates were (2.5±0.1)%, (18.3±0.2)% and (11.6±0.1)% at 24 h, 48 h and 72 h, respectively, P<0.001. The inhibitatory rates of bcl-2 expression were (1.35±0.1)%,(2.2±0.01)% and (5.53±0.01)% at 24 h, 48 h and 72 h, respectively,P<0.005. The morphological changes of apoptosis were fuound. CONCLUSION: Tsα1 can inhibit proliferation of SMMC-7721 cells, induce apoptosis in SMMC-7721 cells and inhibit expression of bcl-2.
【Key words】 liver neoplasms/drug therapy; thymosin/pharmacyology; tumor cells, cultured; apoptosis;
- 【文献出处】 肿瘤防治杂志 ,Chinese Journal of Cancer Prevention and Treatment , 编辑部邮箱 ,2005年23期
- 【分类号】R735.7
- 【被引频次】3
- 【下载频次】56