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Tsα1诱导人肝癌SMMC-7721细胞凋亡和抑制bcl-2基因的表达

Expression of apoptosis and inhibitory bcl-2 in human hepatocelluar cancer SMMC-7721 cells induced by thymope α1

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【作者】 李晓诗金逸杨宗华

【Author】 LI Xiao-shi1, JIN Yi2, YANG Zong-hua21.Departmant of Tumor, Nanshan Hospital of Shenzhen, Shenzhen 518052,P.R.China2.Departmant of Biological Treated, First People’s Hospital of Changshu, Changshu 215500,P.R.China

【机构】 深圳市南山医院肿瘤科常熟市第一人民医院生物治疗室常熟市第一人民医院生物治疗室 广东深圳518052江苏常熟215500

【摘要】 目的:研究胸腺肽α1(Tsα1)在诱导肿瘤细胞凋亡中的作用。方法:应用MTT比色分析法研究Tsα1对人肝癌SMMC7721细胞株的细胞毒作用。流式细胞仪检测法分析Tsα1作用后处于G1期SMMC7721细胞的比率和对凋亡抑制基因bcl2蛋白的抑制率。结果:Tsα1(4000μg/mL)体外培养24、48和72h,对SMMC7721肿瘤细胞抑制率分别是(1.92±0.02)%、(3.27±0.03)%和(7.69±0.03)%,P<0.005;凋亡率分别是(2.5±0.1)%、(18.3±0.2)%和(11.6±0.1)%,P<0.001;bcl2蛋白的抑制率为(1.35±0.1)%、(2.2±0.01)%和(5.53±0.01)%,P<0.005,并呈现细胞凋亡的形态学改变,具有剂量和时间依赖性。结论:Tsα1可直接抑制人肝癌SMMC7721细胞株增殖,抑制bcl2基因表达和诱导肿瘤细胞凋亡。

【Abstract】 OBJECTIVE: To stude the apoptosis induced by thymope α1(Tsα1) in human hepatocelluar cancer SMMC-7721 cells. METHODS: Tsα1 inhibitatory SMMC-7721 cell’s growth by was studied MTT assay; morphological characteristics of apoptosis was observed by microscopy; cell apoptosis rate was measured by flow cytometry and agarrose gel electronphoresis; expression of bcl-2 was detected by flow cytometry. RESULTS: When the concentration of Tsα1 was 4 000 μg/mL, the inhibitatory rates of SMMC-7721 cells were (1.92±0.02)%, (3.27±0.03)% and (7.69±0.03)% at 24 h, 48 h and 72 h, respectively,P<0.005. The apoptosis rates were (2.5±0.1)%, (18.3±0.2)% and (11.6±0.1)% at 24 h, 48 h and 72 h, respectively, P<0.001. The inhibitatory rates of bcl-2 expression were (1.35±0.1)%,(2.2±0.01)% and (5.53±0.01)% at 24 h, 48 h and 72 h, respectively,P<0.005. The morphological changes of apoptosis were fuound. CONCLUSION: Tsα1 can inhibit proliferation of SMMC-7721 cells, induce apoptosis in SMMC-7721 cells and inhibit expression of bcl-2.

  • 【文献出处】 肿瘤防治杂志 ,Chinese Journal of Cancer Prevention and Treatment , 编辑部邮箱 ,2005年23期
  • 【分类号】R735.7
  • 【被引频次】3
  • 【下载频次】56
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