【摘要】 目的:探讨C60-AEDP对H22肝癌细胞和S180肉瘤细胞的影响及作用机制。方法:体外培养H22肝癌细胞和S180肉瘤细胞,MTT法观察药物在不同浓度、不同条件和不同时间下,对细胞生长方式的影响,FCM法了解其作用机制。结果:光照12h,C60-AEDP对H22和S180肿瘤细胞有生长抑制效应,呈浓度效应关系;在非光照条件下,C60-AEDP对H22和S180肿瘤细胞有生长抑制效应,呈浓度-时间效应关系;通过FCM观察,在光照条件下,C60-AEDP可引起G1/S周期阻滞;在非光照条件下,C60-AEDP可引起M/G1周期细胞阻滞;并都可引起细胞凋亡增加,H22、S180肿瘤细胞凋亡率在光照条件下分别增加了32%、26%,而非光照条件下分别增加了9%、5%。结论:在体外条件下,C60-AEDP在光照条件下和非光照条件下,均表现出对肿瘤细胞生长微弱的抑制效应。这种微弱的抑制效应可能是通过对细胞周期的影响而发挥作用。肿瘤防治杂志,2005,12(20):1530-1534更多还原

【Abstract】 OBJECTIVE:To nvestigate the impact and mechanism of C₆₀-AEDP to liver cancer cell H₂₂ and sarcoma cell S₁₈₀. METHODS: H₂₂ and S₁₈₀ cells were incubated at 96-hole board to observe effects of the drug on cell growth by MTT method, and to investigate the mechanism by FCM method. RESULTS: Under the illumination condition for 12 h or not, it was demonstrated that C₆₀-AEDP inhibited the growth of H₂₂ and S₁₈₀ cells, and the inhibitory rate was concentration- and time-dependents. C₆₀-AEDP caused G₁/S cycle block under illumination condition and M/G₁ cycle block without illumination. The apoptosis rates of H₂₂ and S₁₈₀ cells were enhanced in the level of 32%, 26% under illumination condition, and 9%, 5% without illumination respectively. CONCLUSIONS: C₆₀-AEDP has the slightly inhibitory effect on tumour cells of H₂₂ and S₁₈₀. It may be worked by the influence on cell cycle.更多还原