【摘要】 目的探讨广东地区宫颈癌组织中HPV16肿瘤相关性抗原E6基因序列的多态性及同源性。方法采用通用引物PCR直接测序法对宫颈癌标本中的HPV分型,从含有HPV16型的标本中采用自行设计的多重引物通过巢式PCR扩增出HPV16E6,经DNA序列测定法检测其基因变异,进而分析其同源性。结果50例宫颈癌组织HPV-DNA的检出率为78%,其中HPV16和HPV18型混合感染18例,单纯HPV16型感染15例。含有HPV16型的标本34例中扩增出HPV16E625例。其中178位核苷酸变异较大,变异率为72%,其相应氨基酸均由天冬氨酸变为谷氨酸。结论HPV16E6DNA序列发生碱基替换的区域主要在氨基端94~241位,羧基端相对保守,未见变异。广东地区宫颈癌组织中HPV16E6的热点突变为Nt178。更多还原

【Abstract】 OBJECTIVE: To explore the intratypic nucleotide and amino acid sequence variations in the E6 open reading frame of human papillomavirus type16 in cervical cancer tissue. METHODS: The types of HPV was identified by PCR product sequencing. HPV 16E1 gene fragment in cervical cancer tissue was amplified by HPV-specific PCR. RESULTS: The detection rate of high-risk HPV types in 50 csaes of cervical cancers was 78%. The mixed infection of HPV16 and HPV18 was found in 18 cases. The infection of HPV16 alone was seen in 16 cases. HPV16E6 was amplified 25 cases of HPV16E6 anong the total 34 cases. CONCLUSIONS: By sequencing and phylogenetic analysis, the most frequently observed substitution in HPV16E6 ORF are distributed in the discrete regions:94-241. Some substitutions result in non-silent mutation. The hot-spot mutation of HPV16E6 in cervical cancer from Guangdong is found at neolertide 178 in the DNA sequence. Chin J Cancer Prev Treat,2005,12(13):985-988更多还原