【摘要】 合成了新型荧光试剂 1,2_苯并₃,4_二氢咔唑_9乙基对甲苯磺酸酯 ,以此作为柱前衍生化试剂对19种游离脂肪酸 （FFA）进行了优化衍生 .在EclipseXDB -C8色谱柱上 ,采用梯度洗脱对 19种游离脂肪酸（FFA）衍生物进行了完全基线分离及定量分析 .荧光检测激发和发射波长分别为λex=333nm ,λem=390nm .多数脂肪酸的线性回归系数大于 0 .9989,检测限在 2 4 .8～ 80 .37fmol.采用APCI大气压化学电离源的正离子模式 ,实现了土壤和苔藓中FFA组分的定性 .方法具有稳定良好的重现性 ,对实际样品测定结果满意 .更多还原

【Abstract】 A novel fluorescence reagent 1,2-benzo-3,4-dihydrocarbazole-9-ethyl-toluene-4-sulfonate （BDBETS） was synthesized, and using which as pre-column derivatization reagent ,19 fatty acids were derivatized optimally. On a reversed-phased Eclipse XDB-C₈ column, 19 fatty acid derivatives were separated at baseline and analysized quantificationally. Fluorescent detection excitation and emission wavelengths were set at λ_ex 333 nm, λ_em390 nm, respectively. Correlation coefficients for fatty acid derivatives are >0.9989, and detection limits （at signal-to-noise of 3∶1） are 24.8～（80.37） fmol. Qualificative analysis of free fatty acids from soil and bryophyte samples was obtained on the basis of APCI source at positive model detection. The established method is sensitive and reproducible for the determination of free fatty acids from real samples with satisfactory results.更多还原