【摘要】 为了寻找新的抗肿瘤活性化合物,采用硅胶、SephadexLH20柱色谱和高效液相色谱,从白浅灰链霉菌Streptomycesalbogriseolus(A2002)中分离得到3个环二肽;利用理化及波谱学方法鉴定其结构分别为环(丙氨酸缬氨酸)[cyclo(AlaVal),1],环(丙氨酸脯氨酸)[cyclo(ProAla),2]和环(脯氨酸酪氨酸)[cyclo(ProTyr),3]。更多还原

【Abstract】 In the course of our screening for new anti-tumor compounds from marine-derived microbial strains, it was found that the acetonic extract from the fermentation of Streptomyces albogriseolus A2002, a marine-derived actinomycete strain isolated from a Jiaozhou Bay sediment sample, significantly inhibited the cell cycle of tsFT210 cells at the G2/M phase and induced apoptosis in tsFT210 cells. Thus, the chemical examination was undertaken on the constituents of bioactive fractions obtained by bioassay-guided fractionation from the fermentation of producing strains. Compounds 1-3 were isolated by repeated column chromatography over Sephadex LH-20 and silica gel, followed by the prepared HPLC separation. Their structures were identified as cyclic dipeptides, cyclo-(Ala-Val) (1), cyclo-(Ala-Pro) (2) and cyclo-(Pro-Tyr) (3), respectively, by the spectroscopic method.更多还原