【摘要】 目的制备尼群地平脂质体,测定脂质体的包封率。方法采用单相溶液冻干法制备尼群地平脂质体,用超滤法分离脂质体和游离药物,紫外分光光度法测定其含药量和包封率,并考察了冻干产物的稳定性。结果尼群地平脂质体的含药量为(0.60±0.02)g·L-1,包封率为(65.1±2.1)%,长期放置,包封率变化不大。结论采用单相溶液冻干法制备尼群地平脂质体和紫外分光光度法测定脂质体包封率,方法可行。更多还原

【Abstract】 Objective To prepare nitrendipine liposomes,determine its encapsulation efficiency by Ultraviolet spectrophotometry.Methods The nitrendipine liposomes were prepared by freeze-drying of monophase solution.The ultrafiltration method was used to separate the free nitrendipine from liposomes,ultraviolet spectrophotometry method was used to determine its drug content and encapsulation efficiency,and stability of liposomes was studied.Results The drug content of nitrendipine liposomes was(496±10.4) mg L-1.The encapsulation efficiency was(50.25±0.62)%,and it did not change obviously after storage of 12 months.Conclusion It’s feasible to prepare nitrendipine liposomes by freeze-drying of monophase solution and determine its encapsulation efficiency by Ultraviolet spectrophotometry.更多还原