节点文献
烯唑醇、三唑醇和腈菌唑的GC和HPLC分析方法
Study on the Quantitative Analysis Method of Diniconazole, Triadimenol and Myclobutanil by GC and HPLC
【摘要】 采用气相色谱法和液相色谱法两种分析方法分别对烯唑醇、三唑醇和腈菌唑进行定性定量分析。气相色谱法中分别以二十烷和邻苯二甲酸二丁酯为内标物,色谱柱都采用3%OV-101chromosorbAWDMCS150–177μm,1m×3mm玻璃柱,柱温为195℃,汽化温和检测温均为230℃,烯唑醇、三唑醇和腈菌唑的回收率分别为99.5%~101.0%,99.5%~101.2%和98.9%~99.7%,方法变异系数分别为0.44%,0.32%和0.21%。高效液相色谱法中使用Nova-PaKC18250mm×4.6mm色谱柱,以甲醇–水(体积比85:15)为流动相,检测波长为252nm、223nm和223nm。烯唑醇、三唑醇和腈菌唑的回收率分别为98.9%~100.4%,99.4%~100.9%和99.5%~101.2%,方法变异系数分别为0.65%,0.41%和0.47%。
【Abstract】 The quantitative analysis method of diniconazole, triadimenol and myclobutanil with GC and HPLC was studied. In gas chromatographic method, a 3% OV–101 on Chromosorb AW DMCS 150–177 μm, 1 m×3 mm (i.d.) glass column was used at column temperature of 195℃ and detector temperature of 230 ℃ . The internal standard was n-eicosane and dibutyl o-phthalate respectively. This method had a recovery of 99.1%~100.3%, 98.5%~99.8% and 99.3%~100.7%, their coefficients of variation were 0.44%, 0.32% and 0.21%, respectively. Operating conditions by HPLC were C18 column (250 mm×4.6 mm), V(methanol):V(water) = 85:15 as mobile phase, spectrum length UV–252 nm,223 nm and 223 nm. This method had a recovery of 99.1%~100.7%, 98.9%~99.6% and 99.9%~101.2% and their coefficient of variation were 0.65%, 0.41% and 0.47% for diniconazole, triadimenol and myclobutanil respectively.
【Key words】 GC; HPLC; analysis; diniconazole; triadimenol; myclobutanil;
- 【文献出处】 现代农药 ,Modern Agrochemicals , 编辑部邮箱 ,2005年06期
- 【分类号】TQ455
- 【被引频次】22
- 【下载频次】409