【摘要】 以合成的半抗原与牛血清白蛋白(BSA)的联结物为免疫抗原,获得对氯嘧磺隆高亲合力的兔抗血清,建立了氯嘧磺隆的直接竞争酶联免疫检测方法。抗血清效价为6.4×104,IC50值为10.9ng/mL,测定的线性范围是0.4～295.4ng/mL。在河水中的添加回收率为87.9%～115.7%。结构相似的常用磺酰脲类除草剂与氯嘧磺隆抗体的交叉反应率为0.1%～4.8%。所建立的方法可用于河水中氯嘧磺隆的残留检测。更多还原

【Abstract】 A direct competitive enzyme immunoassay for the quantitative analysis of chlorimuron-（ethyl） was developed. Polyclonal antibodies（PAbs） were prepared from rabbits immunized with （hapten-） protein conjugates. The titer of antiserum was 6.4×10⁴,IC₅₀ was 10.9 ng/mL. Chlorimuron-ethyl could be quantitated in the range of 0.4[KG-*3]²95.4 ng/mL.Average recovery of chlorimuron-ethyl in river water was 87.9%[KG-*3]¹15.7%.The cross-reactivity of PAbs of chlorimuron-ethyl with other （analogues） were 0.1%[KG-*3]⁴.8%. This assay can be applied for the quantitation of chlorimuron-ethyl residue in river water.更多还原