【摘要】 为建立棉铃虫Helicoverpaarmigera微粒体P450的分离纯化方案,比较了不同浓度下聚乙二醇 8000(PEG8000)对棉铃虫幼虫中肠和脂肪体微粒体蛋白的沉淀作用。结果表明,终浓度为8.0×104 mg/L的PEG8000可以使中肠和脂肪体微粒体蛋白的 78%沉淀下来,其中包含的细胞色素P450分别占中肠和脂肪体P450总量的 28%和 34%。SDS PAGE显示,中肠微粒体经8.0×104 mg/L的PEG8000沉淀后,被沉淀蛋白的分子质量主要集中在 14.1~ 40kD和 66~97kD范围内。更多还原

【Abstract】 In order to develop a protocol for purifying cytochrome P450 in the cotton bollworm, (Helicoverpa) armigera, the precipitation results of PEG8000 at different (concentration) to microsomal proteins of cotton bollworm were compared. The results showed that at the concentration of (8.0×10~4 mg/L,) 78% microsomal proteins including 28% total P450 of midgut microsome or 34% total P450 of fatbody microsome were precipitated. SDS-PAGE showed that most of the precipitated microsomal proteins from midgut were those which molecular weight was among the range of (14.1~40 kD) and 66~97 kD.更多还原