【摘要】 分别用大肠杆菌(Escherichia coli)表达的猪传染性胸膜肺炎放线杆菌(A ctinobacillus pleuropneum oniae)外毒素(A px)Ⅰ、Ⅱ和Ⅲ为抗原,免疫Balb/c小鼠,经细胞融合、克隆与筛选,分别获得能稳定分泌A pxⅠ、A pxⅡ和A pxⅢ单克隆抗体的杂交瘤2株(4、16)、3株(14、3F11、16G10)和2株(44、53)。这7株杂交瘤细胞的染色体数分别为87(81￣94)、84(81￣88)、90(85￣94)、87(80￣94)、82(79￣85)、93(88￣99)和85(82￣87),其腹水ELISA效价分别为100×210、100×29、100×210、100×27、100×214、100×214和100×212。间接ELISA和W estern blot分析结果证明,所有单克隆抗体均具有与相应天然毒素的反应活性,且特异性良好。更多还原

【Abstract】 Seven hybridoma clones were generated by fusion of the SP2/0 myeloma cells and the splenocytes obtained from Balb/c mice immunized by the recombinant ApxⅠ, ApxⅡ and ApxⅢ of Actionbacillus pleuropneumoniae (APP). Among the 7 hybridoma clones, 2 of them (4 and 16) could secret monoclonal antibodies against ApxI, 3 of them (3F11,16G10 and 14) against ApxⅡ and the rest 2 (44 and 53) against ApxⅢ. Their chromosome numbers were 81~94, 81~88, 85~94, 80~94, 79~85, 88~99 and 82~87, respectively. The ELISA titers of the ascite fluids induced by the 7 hybridomas were 100×210, 100×29, 100×210, 100×27, 100×214, 100×214 and 100×212, respectively. Indirect ELISA and Western blotting analyses indicated that all the 7 strains of monoclonal antibodies could react specifically with the corresponding Apx toxin isolated from the cultures of different APP strains.更多还原