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乳头管灌注内毒素诱发大鼠实验性乳腺炎模型的建立
Establishment of Experimental Mastitis Model by Endotoxin via Teat Duct in Rat
【摘要】 20只泌乳期SD雌性大鼠随机分成正常对照组(n=5)和实验组(n=15),产后72h分别用灭菌生理盐水(PSS)和不同剂量(5、10和50μg)的大肠杆菌(Escherichia coli)内毒素经乳头管灌注到大鼠第4对(腹部)乳腺内,灌注后24h处死大鼠,取乳腺组织固定,进行组织学观察,测定了血清及乳腺组织中肿瘤坏死因子α(TN F-α)、分泌型IgA(sIgA)的含量及N-乙酰-β-D-氨基葡萄糖苷酶(N A G ase)的活力变化。组织病理学观察显示:灌注5和10μg内毒素组的大鼠乳腺组织结构不清,但仍可辨认,腺泡腔内出现嗜中性粒白细胞;灌注50μg内毒素组的大鼠乳腺组织破坏得更为严重,乳腺组织结构消失,乳腺为大量密集嗜中性粒白细胞浸润。与对照组相比,灌注50μg内毒素组大鼠乳腺组织中TN F-α和sIgA含量及N A G ase活力显著升高(P<0.05);实验组大鼠血清中TN F-α含量无显著变化(P>0.05),而N A G ase活力显著低于正常对照组(P<0.05),灌注50μg内毒素组血清中sIgA含量显著高于正常对照组(P<0.05)。结果表明乳头管灌注内毒素诱发大鼠实验型乳腺炎动物模型成功建立。
【Abstract】 Twenty lactating SD rats were randomly divided into control (n=5) and treatment groups (n =15). Either sterile physiological saline solution (PSS) or different amount (5, 10 and 50 μg) Escherichia coli endotoxin was inoculated into the fourth (abdominal) mammary gland via the teat duct 72 h after parturition. Exactly 24 h after inoculation, the rats were euthanatized and the mammary glands were removed and fixed for histopathologic evaluations. Tumor necrosis factor-α (TNF-α) concentration, N-acetyl-β-D-glucosaminidase (NAGase) activity and secretory IgA (sIgA) concentration in the mammary glands and plasma were determined respectively. Histopathologic examination of mammary gland revealed that 5 μg endotoxin-infused mammary glands had pathological changes but alveolar structures were still discernible, numbers of polymorphonuclear neutrophils (PMN) were present in alveoli. Cases of the 10 μg endotoxin-infused mammary glands were similar. While the mammary glands infused with 50 μg endotoxin had more severe pathologic lesion, the normal alveolar architecture was lost and infiltrated with numerous PMNs. In comparison with those of PSS-treated rats, TNF-α and sIgA concentrations, NAGase activity were significantly (P <0.05) higher in 50 μg endotoxin-infused mammary glands. TNF-α concentration in plasma had no remarkable change. There was decrease in NAGase activity in plasma of all endotoxin-treated rats and significant increase (P <0.05) of sIgA concentration in plasma of 50 μg endotoxin-infused rats. The results demonstrated that the rat experimental mastitis model infused with endotoxin via teat duct was successfully constructed.
- 【文献出处】 农业生物技术学报 ,Journal of Agricultural Biotechnology , 编辑部邮箱 ,2005年05期
- 【分类号】S857.26
- 【被引频次】57
- 【下载频次】322