【摘要】 采用植物内生放线菌分离方法从健康番茄(Lycopersiconesculentum)根中分离纯化出58株内生放线菌,从中挑选部分代表性菌株进行代谢产物除草活性检测,发现编号为S5的菌株的代谢产物对小麦(TriticumaestivumL.)和绿豆(Phaseolusra-diatusL.)种子的发芽有强烈的抑制作用,但对发芽后的幼苗生长无明显影响。以百喜草(Paspalumnotatum)和狗牙根(Cynodondactylon)为实验对象,证明S5菌株的代谢产物的确能抑制草籽的发芽,该活性具有潜在的除草效能。经初步鉴定,S5菌株为淡紫灰链霉菌淡青变种(Streptomyceslavendulaevar.glaucescens)。发酵条件实验结果表明,S5菌株在1%葡萄糖和0.3%牛肉膏的S培养基中,以2%接种量在pH7.0和25℃摇床培养,可得到最强的抑制种子发芽的生物活性。更多还原

【Abstract】 Fifty-eight actinomycetes were isolated from tomato(Lycopersicon esculentum) roots. Among these actinomycetes,43 representative strains were screened for their herbicidal activities. Results showed that the strain S5 intensively inhibited germination of wheat (Triticum aestivum) and mung bean (Phaseolus radiatus) seeds as well as grass seeds such as Paspalum notatum and Cynodon dactylon, while it did not suppress growth of the wheat seedlings. The strain S5 was identified as Streptomyces lavendulae var. glaucescens according to their cultural characteristics, morphological characteristics, physiological and biochemical characteristics. When cultivated with 2% inoculation amount in S medium containing 1% glucose, 0.3% beef-extract, pH 7.0, under shaking at 25C, strain S5 showed the highest herbicidal activity.更多还原