【摘要】 根据牛(Bostaurus)主要组织相容复合体(MHC)DRB3基因的已知序列设计引物,对中国牦牛(B.grunniens)的DRB3基因exon2进行扩增和克隆测序,并根据DRB3基因exon2序列对牛亚科的遗传多样性和分子系统发育进行了分析。结果在234的片段中发现有115个多态位点,多态位点百分率为49.15%。由标准遗传距离和净遗传距离构建的牛亚科系统发育树,可将牛亚科分为5个属,支持将牦牛作为牛亚科中一个独立的属的观点。多态性较丰富的DRB3基因exon2适于属间系统发育分析。更多还原

【Abstract】 A pair of primers designed according to the sequences of Bos taurus reported was used to amplifying the exon2 of DRB3 of major histocompatibility complex (MHC) in yak(B. grunniens), and then the exon2 was cloned and sequenced. Sequence variation and phylogeny were analyzed according to the sequences of exon2 of DRB3 of bovine. The result showed that polymorphism of exon2 of DRB3 was rich, and 115 polymorphic sites were observed in sequence of 234 bp, and the percentage of polymorphism loci was 49.15%. Phylogenetic tree constructed by standard genetic distance and net genetic distance suggested that Bovinae were clustered in 5 genera, which supports the idea that B. grunniens is a separate genus. These showed that rich polymorphism of exon2 of MHCDRB3 was suitable for phylogenetic analysis among different genus.更多还原