【摘要】 目的:探讨过敏性紫癜(AP)的遗传背景及免疫学发病机制,为明确AP的病因学及预后提供免疫学方面的资料,同时也为探索其免疫治疗提供有价值的线索。方法:采用分析性研究策略和聚合酶链反应——序列特异性引物分型技术(PCR-SSP)对80例内蒙古地区汉族儿童AP病人和108例正常汉族儿童进行HLA-DRB 1等位基因型别分析。结果:汉族儿童HLA-DRB 1各等位基因均被检出。HLA-DRB 1*010x、HLA-DRB 1*120x的基因频率在AP患儿组较正常对照组明显升高(分别为13.75%vs 4.16%、12.50%vs5.56%,);HLA-DRB 1*080x的基因频率在AP患儿组较正常对照组明显降低(2.50%vs 8.33%)。这些基因频率在两组间的分布经统计学检验差异有显著性。其它HLA-DRB 1等位基因频率在两组间的分布经统计学检验差异无显著性。结论:HLA-DRB 1*010x、HLA-DRB 1*120x与AP的易感性呈正相关,可能是AP的易感基因。HLA-DRB 1*080x与AP的易感性呈负相关,可能是AP的保护基因。更多还原

【Abstract】 Objective: To investigate the correlation of the polymorphism of HLA-DRB1 alleles and Han nationality children patients with anaphylactoid purpura(AP) in Inner Mongolia in order to interpret the genetic basis of susceptibility and immune pathogenesis of AP.Methods: Eighty Han nationality children patients with AP and 108 normal Han nationality children controls in Inner Mongolia Autonomous Region were tested for HLA-DRB1 allelic types analysis by PCR-SSP technique.Results: HLA-DRB1*010x gene frequency and HLA-DRB1*120x gene frequency were significantly higher in patients with AP than in the controls(13.75% vs 4.16%,12.50% vs 5.56% respectively);HLA-DRB1*080x gene frequency was significantly lower in patients with AP than in the controls(2.50% vs 8.33%).Among AP patients and controls,the difference of these gene frequencies had statistical significance.There were no statistically significant differences in other alleles between the two groups.Conclusion: HLA-DRB1*010x and HLA-DRB1*120x are positively correlated with the suspectibility of AP,which are probable the susceptible genes of AP among Han nationality children patients with anaphalactoid purpura in Inner Mongolia,and HLA-DRB1*080x is negatively correlated with the suspectibility of AP,which is probably the protective gene of AP.更多还原