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应用RAPD技术分析绒山羊DNA的多态性

THE GENEFIC DSVERSITY ANALISIS OF CASHMEVE GOAFS DNA BY RAPD TECHNOLOGY

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【Author】 LIANG Yan~1,LIU Ying-chun~1,ZHANG Run-wu~2,ZHOU Huan-min~3(1 College of Animal Science and Medicine,Inner Mongolia Agricultural University,Hohhot 010018,China;2 Kubuqi Desert Institute,Dalate Banner,014300,China3 College of Biology Engineering,Inner Mongolia Argricultural University,Hohhot 010018,China)

【机构】内蒙古农业大学动物科学与医学学院；内蒙古库布齐沙漠研究所；内蒙古农业大学生物工程学院呼和浩特010018；呼和浩特010018；达拉特旗014300；呼和浩特010018；

【摘要】本实验应用RAPD技术,对辽宁多绒山羊、辽宁绒山羊、内蒙古阿尔巴斯白绒山羊和杂种多绒山羊的22个DNA模板进行了多态性比较和遗传距离分析,根据聚类分析构建了系统发生树状图。36条引物中筛选出11条引物具有多态性扩增片段。得到多态性位点59个,占总位点数的90.77%,分析显示杂种多绒山羊和辽宁绒山羊的亲缘关系较近。更多还原

【Abstract】 Random amplified polymorphic DNA(RAPD) analysis was used to estimate genetic diversity and relationship in 22 DNA templates which belong to three Inner Monglia cashmere goat breeds and liaoning cashmere goat breeds.36 primers were used,11primers were screened to generate polymorphic bands.The genetic distance matrix was obtained by proportion of shared fragments.Pendrogram were generated by UPGMA.The results indicated that two Liaoning cashmere goats were clustered together.Kubuqi Cashmere goats was cldosely related to Liaoming cashmere.更多还原

【关键词】绒山羊； RAPD；遗传多样性；
【Key words】 cashmere goats； RAPD； genetic diversity；

【基金】内蒙古自治区教育厅重点领域研究项目

【文献出处】内蒙古农业大学学报(自然科学版) ,Journal of Inner Mongolia Institute of Agriculture and Animal Husbandry , 编辑部邮箱 ,2005年03期

【分类号】S827
【被引频次】4
【下载频次】116

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