【摘要】 目的:探讨慢性肾功能衰竭(CRF)性勃起功能障碍(ED)的发病机制。方法:应用SD雄性大鼠分两期行5/6肾脏切除术,建立CRF动物模型。将假手术组(NCRF组,n=30)、CRF模型大鼠(CRF组,n=45)分别随机均分为Ⅰ(2周)、Ⅱ(4周)、Ⅲ(6周)3组,并分别于2、4、6周注射阿朴吗啡(APO,80μg/kg)后观察大鼠阴茎勃起情况,筛选CRF性ED模型大鼠;测定阴茎海绵体组织一氧化氮合酶(NOS)的活性,及其组织的M asson染色图像分析。结果:CRF性ED大鼠阴茎海绵体组织NOS活性及平滑肌面积显著降低(P<0.01或P<0.05),胶原纤维略有增加,且上述变化与CRF病程密切相关。海绵窦血管腔无明显变化。结论:CRF严重影响阴茎勃起功能,阴茎海绵体组织NOS活性降低及阴茎海绵体平滑肌面积的减少可能是其重要的发病机制。更多还原

【Abstract】 Objective: To investigate the mechanism of erectile dysfunction（ED） in rat models with chronic renal failure（CRF）. Methods: Chronic renal failure was induced by adult male Sprague-Dawley rats,which were subjected to an excisional 5/6 nephrectomy.The rats in NCRF group and CRF group were divided into three groups randomly.Injected with apomorphine（APO,80 μg/kg）,penile erections of three groups were observed and noted by the 2^nd week,4^th week respectively and 6^th week from the 14^th day of 5/6 nephrectomy and experimental models of ED with CRF were selected;NOS activity was examined and the microstructures of penile were observed under optical microscope with computer configuration image analysis system in selected rat models.Results: Compared with the controls,the areas of smooth muscle and NOS activity in the penile cavernous tissue of ED rats with CRF decreased significantly（P<（0.01） or P<（0.05））,and collagen fibers sightly increased,and these alterations had close correlations with the duration of CRF.The areas of blood sinus had no obvious changes. Conclusions: Penile erection is seriously affected in rats with CRF.The decreases of the number of smooth muscles and NOS activity might be the most important factors.更多还原