【摘要】 目的:观察不同年龄SD大鼠脑室管膜下区(SVZ)和海马GDNFmRNA及其受体GFR- α1mRNA表达水平的变化,探讨其在神经发生及脑老化中的作用。方法:用RT PCR方法检测胚胎18d、新生以及1、3、8、12月龄SD大鼠SVZ和海马GDNFmRNA及其受体GFR -α1mRNA的变化。结果:随着年龄增长,SD大鼠SVZ和海马GDNFmRNA及其受体GFR- α1mRNA表达水平逐渐降低。且各年龄组海马表达参数的幅度小于SVZ。结论:GDNF及其受体GFR- α1在神经发育及脑老化进程中起到一定的作用。更多还原

【Abstract】 Objective To investigate the effect of GDNF and GFRα₁ in the subventricular zone and hippocampus on neural precursor proliferation,via analysing the gene expression of GDNFmRNA and GFRα₁mRNA from Sprague-Dawley rats of different ages. Methods The level of GDNFmRNA and GFR-α₁mRNA in the subventricular zone and hippocampus was observed and compared in rats of different age, using semi-quantitative RT-PCR method with β-actin as an inner consultation. Results With the age increased,the gene expression of GDNFmRNA and GFRα₁mRNA in the subventricular zone and hippocampus was obviously decreased, and the gene expression of GDNFmRNA and GFRα₁mRNA in the subventricular outnumbered those of gene expression in hippocampus of different ages. Conclusions The gene expression of GDNFmRNA and GFRα₁mRNA in the subventricular zone and hippocampus obviously decreases with the increase of age. GDNF and GFRα₁ exert important effects on the neural precursor proliferation, the decline of the neuronal compensatory ability and the neural plasticity of the aged brain.更多还原