【摘要】 通过诱导根状茎上的休眠芽萌发,以其茎尖和幼茎段材料为外植体进行组织培养,建立了大花蕙兰(Cymbidium hynridum)的无菌繁殖体系,并筛选出大花蕙兰无菌繁殖的最佳培养基组成。原球茎诱导的较适宜的培养基为MS+1.5 mg.L-16-BA+0.1 mg.L-1KT+0.05 mg.L-1NAA,诱导率达15%;原球茎增殖培养基在诱导培养的基础上添加150 g.L-1香蕉泥,其增殖系数1个月内可达15~35。原球茎是由顶端分生组织后面的薄壁细胞脱分化,形成胚性细胞,经球状胚发育而来。更多还原

【Abstract】 Dormant buds of Cymbidium hynridum were cultured on MS media to establish micropropagation system.The suitable Protocorm-like bodies inducing medium was MS+1.5 mg·L^-16-BA+0.1 mg·L^-1KT+0.05 mg·L^-1NAA.Induction rate of PLBs is up to 15%.The secondary culture medium of PLBs is MS+1.5 mg·L^-16-BA+0.1 mg·L¹KT+0.05 mg·L^-1NAA +banaba juice150 g·L^-1.The PLBs are developed from the apical meristem of the apical meristem which can grow into seedlings.更多还原