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马立克氏病病毒超强毒UL49基因siRNA表达质粒的构建与鉴定

Construction and identification of siRNA expression plasmid aimed at UL49 gene of Marek′s disease virus RB1B strain

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【Author】 MIAO De-nian~1,WANG Xiu-hua~2,FENG Jiang-nan~3,YAO Hui-juan~1,WU Qiong-shan~3,FAN Sheng-chao~1*,CHEN Pu-yan~2* (1.Animal Husbandry and Veterinary Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai 201106,China;2.Key Laboratory of Animal Disease Diagnostic and Immunology,Ministry of Agriculture,Nanjing Agricultural University,Nanjing 210095,China;3.Wuhan Genesil Biotechnology Co.Ltd.,Wuhan 430074,China)

【机构】上海市农业科学院畜牧兽医研究所；南京农业大学农业部动物疫病诊断与免疫重点开放实验室；武汉市晶赛生物工程技术有限公司；南京农业大学农业部动物疫病诊断与免疫重点开放实验室上海201106；江苏南京210095；湖北武汉430074；上海201106；江苏南京210095；

【摘要】以马立克氏病病毒超强毒(RB1B株)UL49为靶基因,利用计算机辅助设计UL49基因特异的siRNA,再定向克隆至pSilencerTM2.1U6neovector载体中构建siRNA表达重组体,转化DH5α菌株,提取质粒酶切鉴定后,进行测序分析,证实为所需序列。以上结果表明,针对马立克氏病超强毒UL49基因的siRNA表达质粒已构建成功。更多还原

【Abstract】 To seek new gene therapy method of Marek′s disease,siRNAs aimed to UL49 gene of Marek′s disease virus RB1B strain were designed and directionally cloned into pSilencer~TM 2.1-U6 neo vector,and transformed into E.coli DH5α strains.The combinant plasmids were identified by restriction enzyme and sequence analysis.Finally,the plasimds contained siRNA expressing cassette were obtained successfully and made it possible to search gene therapy method of the Marek′s disease.更多还原

【关键词】马立克氏病； RB1B株； UL49基因； siRNA；表达质粒；
【Key words】 Marek′s disease virus； RB1B strain； UL49 gene； siRNA； expression plasmid；

【基金】上海市启明星计划项目(03QC14041)

【文献出处】南京农业大学学报 ,Journal of Nanjing Agricultural University , 编辑部邮箱 ,2005年03期

【分类号】Q782
【被引频次】6
【下载频次】116

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