【摘要】 研究了里氏木霉β-聚糖酶的生物合成,并比较了以玉米芯、纸浆、粗木聚糖为碳源对里氏木霉β-聚糖酶诱导合成的影响。结果表明:里氏木霉以15 g/L的玉米芯为碳源合成β-聚糖酶,培养4 d时滤纸酶活、羧基纤维素(CMC)酶活、纤维二糖酶活和木聚糖酶活分别为1.03 FPIU/mL、0.54、0.08和149.7 IU/mL;以纸浆为碳源,可得到较高纤维素酶活、较低木聚糖酶活的β-聚糖酶;以粗木聚糖为碳源,可制备低纤维素酶活的木聚糖酶,木聚糖酶活与CMC酶活的比值高达785.4,适合于纸浆的生物漂白。更多还原

【Abstract】 The effects of carbon sources on the preparation of βglycanases were investigated.The result showed: The filter paper activity,CMCase activity,cellobiase activity and xylanase activity were 1.03FPIU/mL,0.54IU/mL,0.08IU/mL and 149.7IU/mL,respectively,when 15g/L corncob employed as carbon source by Trichoderma reesei.The poor-xylanase cellulase was synthesized under 15g/L kraft pulp as carbon source.The poor-cellulase xylanase,which was suitable for biobleaching,was formatted when the T.reesei cultivated in the same concentration of unpurified xylan,and the ratio of xylanase to CMCase was 785.4.更多还原