【摘要】 以接近于LD50的马拉硫磷处理东亚飞蝗Locustamigratoriamanilensis(Meyen)天津北大港和山东无棣种群个体,用SSRP-PCR标记技术分析检验东亚飞蝗在不同选择压力下,存活个体与死亡个体的基因型与马拉硫磷致死作用的可能相关关系。结果表明,无棣种群死亡组的多态性明显高于存活组,而北大港种群存活组的多态性高于死亡组,北大港群体的多态性高于无棣群体。各多态位点与马拉硫磷致死性相关性检测表明:第一,相同引物的不同位点对马拉硫磷的敏感性不同,不同种群对马拉硫磷致死性存在相关性的位点不同;第二,不同处理的东亚飞蝗在同一引物扩增出的同一位点对马拉硫磷毒性的反应不同。这些结果表明,马拉硫磷对东亚飞蝗在不同引物扩增的不同位点有选择致死作用。Nei’s遗传距离值在两个种群中都是在死亡组和存活组最高,表明马拉硫磷对东亚飞蝗种群的遗传结构产生了一定的分化作用。更多还原

【Abstract】 Microsatellite primer PCR technique was used to confirm the relationship between the mortality and polymorphic loci of Locusta migratoria manilensis (Meyen) in two populations under different stress, where all individuals were acutely exposed, respectively, to two different concentrations of malathion nearly to LD50 in 24 hours. It was noted that dead group of Shandongwudi population had higher polymorphism than the surviving group while, it was reversed for Tianjinbeidagang population. Different loci of same primer had different mortality under stress of malathion, namely, in Shandongwudi population only some loci such as for 33.15 core sequence and(GATA)4 showed this trend, revealing that different population had different correlative loci with malathion. Moreover, the x2 test showed a significant difference in correlation between the polymorphic loci and mortality in different treatment such as for 33.15 core sequence at 1 000 bp loci.. Nei’s genetic distance indicated that the genetic distance between the dead group and surviving group was in the highest in two populations and the acute exposure to malathion did cause differentiation in genetic composition at population level in Locusta migratoria manilensis.更多还原