【摘要】 通过在饲料中分别加入不同浓度(铜元素的添加浓度分别为0、7.5、15、30、45mg·kg-1饲料)的硫酸铜和蛋氨酸铜,比较了硫酸铜和蛋氨酸铜对凡纳对虾生长、血液免疫因子及虾体铜的影响。添加蛋氨酸铜的各实验组生长速度同对照组之间没有显著差异;而添加硫酸铜的各组,只有铜添加量为30mg·kg-1饲料的试验组生长速度显著高于对照组(P<0.05),其它组同对照组之间差异不显著;成活率各组间无显著差异。除了铜添加量为15mg·kg-1饲料的试验组,各试验组对虾血清的酚氧化酶(PO)活力都高于对照组,其中当铜添加量为30mg·kg-1时最强,在此时蛋氨酸铜添加组的PO活力高于硫酸铜;随着铜添加浓度的提高,超氧化物歧化酶(SOD)活力增强,在添加量达到30mg·kg-1饲料时达到最大,此时硫酸铜组和蛋氨酸铜组间无差异,都为507。对虾肌肉中铜的含量各组间无显著差异,为(4.07±1.80)mg·kg-1,个体间差异非常大;肝胰脏中铜的最高含量为210.36mg·kg-1,最低为33.78mg·kg-1(空白对照组),明显高于肌肉中的含量。结果表明,在试验条件下,同硫酸铜相比,蛋氨酸铜没有显著的促生长效果,对肌肉、肝铜含量以及SOD活性没有显著影响。更多还原

【Abstract】 In this paper, the effects of Met-Cu and copper sulfate application in the feed on the growth rate, immunological factors and the tissue copper of Litopenaeus vannamei were studied. The supplemental concentration of copper were 0, 7.5, 15, 30, 45 mg·kg^-1.There were not significant differences among the groups supplemented with Met-Cu as well as the control group; the shrimp fed with the feed supplemented with 30 mg·kg^-1 copper sulfate grew faster than the other groups significantly. The differences of the survival rate were not significant among various groups. PO and SOD activity were influenced by the content of feed copper, they were highest when the supplemental copper concentration was 30 mg·kg^-1 feed. There were not significant differences among the shrimp muscle copper content of different groups, the average value was （4.07±1.80）mg·kg^-1, which varied obviously among the individuals. The highest content of the hepatopanreas copper was 210.36 mg·kg^-1, the lowest was 33.78 mg·kg^-1（control group）, it was higher than that in the muscle. The results showed that Met-Cu application in the feed did not enhance the growth or influence the copper content of the muscle and hepatopancreas as well as the SOD activity compared to the copper sulfate under this experiment condition.更多还原