【摘要】 目的探讨尿脱落细胞端粒酶活性检测在膀胱癌早期诊断中的临床意义。方法采用TRAPPCRELISA法检测32例膀胱癌患者尿液及14例非膀胱肿瘤患者尿液脱落细胞的端粒酶活性,并行尿脱落细胞学检查。结果32例膀胱癌患者尿脱落细胞端粒酶活性阳性率为65.6%,14例非膀胱肿瘤患者尿脱落细胞仅1例端粒酶活性阳性。膀胱癌与非膀胱肿瘤患者的端粒酶活性比较有显著性差异(P<0.01),但与膀胱肿瘤的分级、分期无明显相关性(P>0.05),尿脱落细胞端粒酶活性检测的敏感性明显高于尿细胞学检查。结论尿脱落细胞端粒酶活性检测敏感性较高,可用于膀胱肿瘤的早期诊断和术后随访。更多还原

【Abstract】 Objective To investigate the clinical significance of detection of telomerase activity in exfoliated cells in urine for the early diagnosis of bladder carcinoma.Methods Using TRAP-PCR-ELISA assay for the presence of telomerase activity in the 32 cases urine exfoliated cells of bladder cancer.14 cases non bladder tumor as control.Results The positive expression rate of 32 cases telomerase activity were 65.6% in 32 cases,only 1 in 14 non-bladder tumor is positive.The expression of telomerase activity wasn’t related to the grades and phases of bladder carcinoma (P>0.05).Conclusion TRAP-ELISA determination of telomerase activity is more sensitive and specific than other assays in bladder carcinoma,and it would be a good early diagnostic and follow-up maker for bladder tumor.更多还原