【摘要】 用Inter-简单重复序列(ISSR)标记对樟子松东北分布区9个种源的135 个个体遗传变异进行了检测。10 个ISSR 引物扩增共检测到108 个位点,其中,多态位点百分率差异明显,范围在27%～54%之间,多态位点百分率最高的是红花尔基,最低的是卡伦山。物种水平的Shannon 多样性指数(I)为0.1581,Nei 基因多样度(h)为0.2393。遗传变异在种源间占39.65%,在种源内占60.35%。根据参试樟子松9 个种源的聚类图,结果樟子松自然分布区划为2 个种源区:大、小兴安岭种源区;呼伦贝尔种源区。图2 表3 参18。更多还原

【Abstract】 Inter Simple Sequence Repeat (ISSR) was used to detect genetic variation among nine provenances, including 135 individuals. A total of 108 loci were amplified using 10 random primers. The differentiation of the percentage of polymorphic bands (PPB) among differ- ent provenances was evident, ranging from 27% to 54%, of which Honghuaerji provenance had the highest PPB and Kalunshan provenance had the lowest PPB. Shannon’s Information index (I) at species level was 0.1581 and Nei’s gene diversity (h) was 0.2393. Coefficient of gene differentiation (GST) calculated by Popgene was 0.3965, these results indicated that majority of genetic variation (60.35%) was found within provenances. According to dendrogram among pinus sylvestriv provenances, nine provenances were divided into two provenance ar- eas, namely Daxing’an and Xiaoxing’an Mountains provenance area and Hulunbeier provenance area.更多还原