【摘要】 为研究白桦雌花序花期基因表达情况,以两天为间隔对其进行取样。基于SMART策略,通过RT-PCR,将源自最后时期样品的cDNA作为DrivercDNA,源自其他时期样品的cDNA作为TestercDNA,构建抑制性消减文库。EST序列经blastX分析表明,该文库中的基因大致可以归为五类,分别同代谢、物质运输和信号转导、细胞周期、胁迫反应及调控相关。本文对基因表达同发育的关系做了探讨。图4参25。更多还原

【Abstract】 Female inflorescence of Betula platyphylla was sampled at an interval of each two days to analyze the background of gene expression in floral phase. On the basis of SMART strategy, the driver cDNA was obtained from total RNA of the last sample and the tester cDNA was from that of the others by RT-PCR which were subsequently used to construct a subtracted cDNA library. The result of the ESTs (expression sequence tags) blastX showed that the genes in the subtracted cDNA library could be mainly clustered into 5 groups related to metabolism, transportation and signal transduction, cell cycle, stress response, and regulation. The relationship between gene expression and development was also discussed.更多还原