【摘要】 以玉露桃和湖景蜜露桃幼胚及幼胚子叶为试材,研究基本培养基类型、激素、损伤方式等因素对再生的影响。结果表明:培养基是影响幼胚诱导愈伤组织的最重要因素,MS适合供试材料诱导愈伤组织;激素诱导愈伤组织因品种而异,NAA1.0mg·L-1对45d、0.5mg·L-1对55d玉露,BA0.5mg·L-1对55d湖景蜜露效果较好,在试验浓度范围内2,4-D对供试幼胚均无明显效果;幼胚的发育状态是影响诱导愈伤组织的另一因素,玉露桃45d愈伤组织诱导率最高,达96.6%,而55d诱导率为81.6%。愈伤组织可在MS+0.05mg·L-1NAA+1.0mg·L-1BA培养基上分化成芽,再生芽在1/2MS+1.0mg·L-1IBA培养基上生根。幼胚子叶不定芽再生培养基的激素配比为NAA0.50mg·L-1+BA10.0mg·L-1、NAA0.05mg·L-1+TDZ3.0mg·L-1;带胚芽子叶纵向刻伤再生率高;不定梢(芽)在1/2MS+2%蔗糖+7.5g·L-1琼脂+IBA1.0mg·L-1+Ad20mg·L-1的培养基上能生根。更多还原

【Abstract】 The main composition of culture media such as the basic medium and hormones were tested for their effects on Peach ( Prunus persica ) callus frequency from immature embryos (which picked after full flower 45 days and 55 days respectively) under (26±1)?℃ without light in an orthogonal experiment. And a method for plant regeneration from immature cotyledons (55 d) has been developed by cultured on MS media supplemented with 2% sucrose and 0.75% agar and hormone in various combinations. The results showed that: The basal medium was the most important factor for inducing callus from embryos. MS was adapted to ‘Yulu’ (45 d and 55 d) and ‘Hujingmilu’ (55 d). NAA 1.0 mg·L + {-1 } and 0.5 mg·L -1 were better for ‘Yulu’ (45 d and 55 d,respectively), BA 0.5 mg·L -1 was better for ‘Hujingmilu’ (55 d), but 2,4-D hadn’t any important effect on callus inducing from the examined material. Days after fecundation was another factor effect on callus induction. The highest callus frequency from 45 d immature embryos was 96.6% and it was 81.6% from 55 d embryos. Shoots regeneration occurred when callus was transferred to a medium in which the NAA concentration was 0.05 mg·L -1 and BA was 1.0 mg·L -1. Shoot regenerated roots in the medium of 1/2 MS+1.0 mg·L -1 IBA. 2 types of hormone combinations (NAA 0.50 mg·L -1+BA 10.0 mg·L -1, NAA 0.05 mg·L -1+TDZ 3.0 mg·L -1) were favorable to shoots differentiation for the immature cotyledons. The regeneration was changed when cotyledons were wounded horizontally by a razor blade. The best results were obtained when cotyledons with embryonic axis and wounded in the surface. Adventitious shoot could regenerate on the 1/2MS medium with IBA 1.0 mg·L -1 and Ad 20 mg·L -1.更多还原