【摘要】 给昆明种雄性小鼠口服印楝油氯仿提取物2g·kg-1d-1,连续1周,停药后第3周取材,研究了其抗生育作用。结果表明小鼠口服印楝油氯仿提取物后抗生育作用肯定;半数致死量为33g·kg-1;血清中转氨酶的活性在停药后的1～3个月升高,停药后的4～6个月基本恢复至正常,血清中睾丸酮含量在服药前后,无明显改变;流式细胞术研究结果表明:印楝油氯仿提取物引起睾丸组织生精细胞减少,细胞凋亡率升高;对变态期精子细胞核蛋白转换的影响表现为总碱性核蛋白含量减少47.11%,总组蛋白精核蛋白比值升高82.25%。研究表明,印楝油氯仿提取物有较好的抗生育作用,其作用机制主要是干扰精子核蛋白的转换,另外还可能与细胞免疫介导有关,对于控制鼠害有一定的意义。更多还原

【Abstract】 The study was undertaken to evaluate the anti_fertility effect of the extract chloroform isolated from neem oil. Adult male Kunming mice received 2 g\5kg^-1 the extract chloroform orally for one week. The data from fertility tests showed that the extracts chloroform from neem oil has positive antifertility effect,the LD₅₀ is 33 g·kg^-1, the level of aminotransferase increase in first to third months after treated with extracts chloroform, but turn back gradually in 4-6th months, serum testosterone level has no obviously alteration. The flow cytometry data revealed that extract chloroform could cause a decrease in seminiferous cells number,and an increase in the apoptosis rate. The concentration of total alkaline nuclear protein （TNBP ） was decreased by 47.11% and the ratio of total tissue protein （TH）/ spermic nuclear protein （RP） was increased by 80.25%. The action of anti_fertility may be relevant with interferring the mediated immunity in the metamorphous stage. It is showed that a new way method of controling mouse calamity can be developed.更多还原