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银杏胚轴、子叶诱导胚状体发生及成苗的研究
Study on Somatic Embryogenesis of Embryonic Axis and Cotyledon in Ginkgo biloba and Plantlet Formation
【Abstract】 Embryonic axis and cotyledon of immature and mature embryos were induced as explants for embryogenesis in Ginkgo biloba. Results showed somatic embryos could be induced only through embryonic axis and cotyledon of immature embryos. MK + NAA 1 mg·L -1 + 6_BA 1 mg·L -1 was the best medium for somatic embryogenesis from embryonic axis, and somatic embryogenesis rate was 45.28%. MK + NAA 1.5 mg·L -1 + 6_BA 1 mg·L -1 was the best medium for somatic embryogenesis from cotyledon, and somatic embryogenesis rate was 12.9%. The growth and development of somatic embryos were promoted by adding 10% coconut milk into MK, and 34.48% somatic embryos could develop into plants.
【关键词】 银杏;
胚轴;
子叶;
胚状体发生;
植株再生;
【Key words】 Ginkgo biloba; embryonic axis; cotyledon; somatic embryogenesis; plant generation;
【Key words】 Ginkgo biloba; embryonic axis; cotyledon; somatic embryogenesis; plant generation;
【基金】 国家自然科学基金资助项目 ( 3 0 0 70 63 4)
- 【文献出处】 林业科学 ,Scientia Silvae Sinicae , 编辑部邮箱 ,2005年02期
- 【分类号】S718
- 【被引频次】19
- 【下载频次】334