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银杏胚轴、子叶诱导胚状体发生及成苗的研究

Study on Somatic Embryogenesis of Embryonic Axis and Cotyledon in Ginkgo biloba and Plantlet Formation

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【作者】 郭长禄陈力耕何新华Syed Asgar Tori袁海英

【Author】 Guo Changlu 1,2 Chen Ligeng 1 He Xinhua 1,3 Syed Asgar Tori 1 Yuan Haiying 1 (1.Key Laboratory of Ministry of Agriculture for Growth Development and Biotechnology of Horticultural Plant, Zhejiang University Hangzhou 310029; 2.School of the Ocean,Harbin institute of technology Weihai 264209;3.Agricultural College of Guangxi University Nanning 530005)

【机构】 浙江大学农业部园艺植物生长发育与生物技术重点开放实验室浙江大学农业部园艺植物生长发育与生物技术重点开放实验室 杭州310029哈尔滨工业大学海洋学院威海264209杭州310029杭州310029广西大学农学院南宁530005

【Abstract】 Embryonic axis and cotyledon of immature and mature embryos were induced as explants for embryogenesis in Ginkgo biloba. Results showed somatic embryos could be induced only through embryonic axis and cotyledon of immature embryos. MK + NAA 1 mg·L -1 + 6_BA 1 mg·L -1 was the best medium for somatic embryogenesis from embryonic axis, and somatic embryogenesis rate was 45.28%. MK + NAA 1.5 mg·L -1 + 6_BA 1 mg·L -1 was the best medium for somatic embryogenesis from cotyledon, and somatic embryogenesis rate was 12.9%. The growth and development of somatic embryos were promoted by adding 10% coconut milk into MK, and 34.48% somatic embryos could develop into plants.

【基金】 国家自然科学基金资助项目 ( 3 0 0 70 63 4)
  • 【文献出处】 林业科学 ,Scientia Silvae Sinicae , 编辑部邮箱 ,2005年02期
  • 【分类号】S718
  • 【被引频次】19
  • 【下载频次】334
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