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核桃蛋白最佳酶解条件研究

Study on Optimum Condition of Enzymatic Hydrolysis on Walnut Protein

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【作者】 丁晓雯张庆祝乔海鸥

【Author】 DING Xiao-wen, ZHANG Qing-zhu,QIAO Hai-ou (College of Food Science, Southwest Agricultural University, Chongqing 400716, China)

【机构】 西南农业大学食品科学学院西南农业大学食品科学学院 重庆 400716重庆 400716重庆 400716

【摘要】 以水解度为指标,研究五种蛋白酶对核桃蛋白水解作用;结果表明,AS.1398中性蛋白酶为水解核桃蛋白最佳酶,最佳酶解条件为:底物浓度2.5%,E/S为7%,温度40℃,pH7.5,酶解3h;在该实验条件下,AS.1398中性蛋白酶酶解核桃蛋白水解度可达45.37%,水解液仅微苦。

【Abstract】 Studied hydrolysis on walnut protein with 5 kinds of protease. The result showed that AS. 1398 proteinase was the optimum proteinase. The optimum hydrolytic condition were that concentration of walnut protein was 2.5%, E/S was 7%, temperature was 40℃, pH was 7.5, hydrolytic time was 3 hour. Under this experimental condition, hydrolytic degree of walnut protein was 45.37%, the hydrolyzate has only slight bitter.

【关键词】 核桃蛋白蛋白酶水解
【Key words】 walnut proteinproteinasehydrolysis
  • 【文献出处】 粮食与油脂 ,Journal of Cereals & Oils , 编辑部邮箱 ,2005年10期
  • 【分类号】TS201.21
  • 【被引频次】19
  • 【下载频次】447
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