【摘要】 目的探讨食管鳞癌中血管内皮细胞生长因子(vascu lar endothelial growth factor,VEGF)与肿瘤浸润性树突状细胞(tumor infiltrated dendritic cell,TIDC)的关系。方法运用原位杂交和免疫组化方法检测食管鳞癌组织中VEGF蛋白和mR-NA的表达、S-100蛋白标记的TIDC的密度。结果(1)46例食管鳞癌组织中VEGF蛋白在不同浸润深度、不同转移状态的肿瘤中表达阳性率差异有显著性。(2)食管鳞癌中S-100蛋白阳性树突状细胞(dendritic cell,DC)平均密度为(19.83±13.91)个/HP;不同分化肿瘤之间S-100蛋白阳性TIDC密度分别为(18.50±17.27)个/HPF、(28.08±14.05)个/HPF、(15.740±10.38)个/HPF,差异有显著性;不同浸润深度的肿瘤之间TIDC密度分别为(32.78±16.60)个/HPF、(15.20±11.96)个/HPF、(19.83±13.92)个/HPF,差异有显著性;VEGF阳性组TIDC密度为(14.93±12.83)个/HPF,显著低于VEGF阴性组的(26.79±12.61)个/HPF,VEGF表达与DC密度之间存在显著的负相关性(r=-0.462)。结论(1)VEGF蛋白表达和食管鳞癌浸润转移之间有一定的相关性。(2)首次探讨了食管鳞癌中DC密度与VEGF蛋白表达之间的关系,发现VEGF影响鳞癌组织中DC的密度,可能影响宿主的抗肿瘤免疫能力和肿瘤的浸润转移。更多还原

【Abstract】 Purpose To investigate the relation between VEGF and tumor infiltrated dendritic cells in esophageal squamous cell carcinoma.Methods The expression of VEGF and infiltration of dendritic cells in forty-six specimens of esophageal squamous cell carcinoma were examined by immunohistochemical staining(S-P method) and in situ hybridization,using anti-VEGF monoclonal antibody,and anti-S-100 polyclonal antibody and labeled VEGF mRNA probes.Results There was remarkable difference of VEGF protein expression in esophageal squamous cell carcinoma in different situation of invasion and metastasis.The mean density of S-100 positive dendritic cells in esophageal squamous cell carcinoma was 19.83±13.91 per HPF.The densities of S-100 positive dendritic cells in different grade tumor were 18.50±17.27,28.08±14.05 and 15.740±10.38 per HPF,while the densities in different invasion situation were 32.78±16.60,15.20±11.96,19.83±13.92 per HPF,respectively.There were a significant difference in the density of dendritic cells in tumor with different invasion and metastasis.The densities of dendritic cells of VEGF positive expression tumors(14.93±12.83/HPF) were much lower than the tumor of negative expression(26.79±12.61/HPF).There was a close negative relation between the expression of VEGF and the density of dendritic cells in esophageal squamous cell carcinoma(r=-0.462).Conclusions VEGF protein is associated with the invasion and metastasis of esophageal squamous cell carcinoma.It is firstly detected the relation of TIDC and VEGF protein,suggesting that VEGF protein affects the density of TIDC and might affect the immune system and the invasion and metastasis of esophageal squamous cell carcinoma.更多还原