【摘要】 目的探讨p14ARF、p53及FHIT蛋白在甲状腺肿瘤组织中的表达及其意义。方法采用免疫组化SP法检测20例甲状腺腺瘤和28例甲状腺癌组织中p14ARF、p53及FHIT蛋白的表达。结果p14ARF、p53及FHIT蛋白在甲状腺腺瘤和甲状腺癌中阳性率分别为90%、35.7%、15%和75%、90%、7.14%,3种蛋白在甲状腺腺瘤及甲状腺癌的表达差异均有显著性(P<0.05)。p14ARF、p53及FHIT蛋白的表达在甲状腺滤泡癌(FTC)与腺瘤之间,乳头状癌(PTC)与腺瘤之间差异均有显著性(P<0.05),p53及FHIT的表达在髓样癌(MTC)与腺瘤间差异也有显著性(P<0.05)。p14ARF、p53及FHIT蛋白的表达与甲状腺肿瘤的恶性进程有关,与患者年龄、性别及淋巴结转移无关。另外p14ARF与FHIT蛋白的表达呈正相关,并且它们与p53的表达均呈负相关。结论肿瘤抑制蛋白p14ARF和FHIT的缺失以及p53蛋白的高表达可能是甲状腺肿瘤发生的重要原因之一。联合检测p14ARF、p53及FHIT蛋白有助于区分甲状腺腺瘤和甲状腺滤泡癌。更多还原

【Abstract】 Purpose To investigate the expression and value of p14~ARF, p53 and FHIT protein in thyroid tumor tissues. Methods The expression of p14~ARF, p53 and FHIT protein in 20 cases of thyroid adenoma and 28 cases of thyroid carcinoma were detected by S-P immunohistochemical technique. Results Positive rates of p14~ARF, p53 and FHIT were 90%, 15% and 90% in thyroid adenomas and 35.7%,75% and 7.14% in thyroid carcinomas,respectively. The expression of all the three proteins had significant difference between thyroid adenomas and carcinomas(P<0.05), and so did between thyroid follicular carcinoma and adenoma, and thyroid papillary carcinoma and adenoma (P<0.05). The expression of p53 and FHIT were also significantly different between thyroid medullary carcinoma and adenoma. The expression of p14~ARF, p53 and FHIT was related to the malignant progression of thyroid tumors,but they were not correlated with patients’ age, gender and lymph nodes metastasis. Besides, the expression of p14~ARF was positively related to that of FHIT, but negatively related to that of p53. Conclusions Loss of the expression of p14~ARF and FHIT and the over-expression of p53 are one of the important factors in the carcinogenesis of thyroid tumors. Combined immunohistochemical detection of p14~ARF, p53 and FHIT is helpful in distinction of thyroid follicular carcinoma from thyroid adenoma.更多还原